HA and TS designed the research study. TM, YB and HA performed the research. YB, HA, and MA conducted experiments and analyzed the data. HA wrote the first draft of the manuscript. All authors contributed to editorial revisions of the manuscript. All authors read and approved the final version of the manuscript. All authors have participated sufficiently in the work and agreed to be accountable for all aspects of the work. The late Professor TS contributed to the conception and execution of this study. His scientific insight and dedication were instrumental, and this work is respectfully dedicated to his memory.
The study was conducted in accordance with the guidelines of the Declaration of Helsinki. The protocol was approved by the institutional ethics review board of Gifu University (Certificate No. 2019-283). Written informed consent was obtained from all participants.
Not applicable.
This research received no external funding.
The authors declare no conflict of interest.
During the preparation of this work, the authors used AI assistance to check spelling and grammar. After that, the authors reviewed and edited the content as needed and take full responsibility for the content of the publication.